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Image Search Results
Journal: Cancer
Article Title: Colorectal carcinoma cell production of transforming growth factor beta decreases expression of endothelial cell vascular endothelial growth factor receptor 2.
doi: 10.1002/cncr.26247
Figure Lengend Snippet: Figure 6. (A) Representative Western blot shows vascular endothelial growth factor receptor 2 (VEGFR2) and activated Smad protein expression and (B) proportion of VEGFR2 protein levels in vehicle-treated and 5 ng/mL transforming growth factor beta (TGF-b)-treated bovine aortic endothelial cells (BAECs) cultured in atmospheric (21%) O2 and <0.1% O2 (anoxia) over 6 and 24 hours. Analysis of variance, P ¼ .0015; n ¼ 4; *P < .05. (C) VEGFR2 and Smad protein levels in BAECs treated for 24 hours with 0 to 50 ng/mL bone morphogenetic protein 9 (BMP9) are shown. (D) VEGFR2 and Smad protein levels in BAECs treated for 24 hours with combinations of 10 ng/mL BMP9, 5 ng/mL TGF-b1, and 5 lM SB-431542 (SB) are shown. Western blot is representative of 2 independent experiments.
Article Snippet: BAECs were grown until confluent and serum-starved overnight before treatment in serum-free medium with 0.1 to 20 ng/mL of recombinant human TGF-b1; 10 ng/mL
Techniques: Western Blot, Expressing, Cell Culture