mini protein trans blot apparatus Search Results


95
R&D Systems recombinant human bmp9
Figure 6. (A) Representative Western blot shows vascular endothelial growth factor receptor 2 (VEGFR2) and activated Smad protein expression and (B) proportion of VEGFR2 protein levels in vehicle-treated and 5 ng/mL transforming growth factor beta (TGF-b)-treated bovine aortic endothelial cells (BAECs) cultured in atmospheric (21%) O2 and <0.1% O2 (anoxia) over 6 and 24 hours. Analysis of variance, P ¼ .0015; n ¼ 4; *P < .05. (C) VEGFR2 and Smad protein levels in BAECs treated for 24 hours with 0 to 50 ng/mL bone morphogenetic protein 9 <t>(BMP9)</t> are shown. (D) VEGFR2 and Smad protein levels in BAECs treated for 24 hours with combinations of 10 ng/mL BMP9, 5 ng/mL TGF-b1, and 5 lM SB-431542 (SB) are shown. Western blot is representative of 2 independent experiments.
Recombinant Human Bmp9, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96
Bio-Rad mini trans blot cell
Figure 6. (A) Representative Western blot shows vascular endothelial growth factor receptor 2 (VEGFR2) and activated Smad protein expression and (B) proportion of VEGFR2 protein levels in vehicle-treated and 5 ng/mL transforming growth factor beta (TGF-b)-treated bovine aortic endothelial cells (BAECs) cultured in atmospheric (21%) O2 and <0.1% O2 (anoxia) over 6 and 24 hours. Analysis of variance, P ¼ .0015; n ¼ 4; *P < .05. (C) VEGFR2 and Smad protein levels in BAECs treated for 24 hours with 0 to 50 ng/mL bone morphogenetic protein 9 <t>(BMP9)</t> are shown. (D) VEGFR2 and Smad protein levels in BAECs treated for 24 hours with combinations of 10 ng/mL BMP9, 5 ng/mL TGF-b1, and 5 lM SB-431542 (SB) are shown. Western blot is representative of 2 independent experiments.
Mini Trans Blot Cell, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Bio-Rad slot-blot apparatus
Figure 6. (A) Representative Western blot shows vascular endothelial growth factor receptor 2 (VEGFR2) and activated Smad protein expression and (B) proportion of VEGFR2 protein levels in vehicle-treated and 5 ng/mL transforming growth factor beta (TGF-b)-treated bovine aortic endothelial cells (BAECs) cultured in atmospheric (21%) O2 and <0.1% O2 (anoxia) over 6 and 24 hours. Analysis of variance, P ¼ .0015; n ¼ 4; *P < .05. (C) VEGFR2 and Smad protein levels in BAECs treated for 24 hours with 0 to 50 ng/mL bone morphogenetic protein 9 <t>(BMP9)</t> are shown. (D) VEGFR2 and Smad protein levels in BAECs treated for 24 hours with combinations of 10 ng/mL BMP9, 5 ng/mL TGF-b1, and 5 lM SB-431542 (SB) are shown. Western blot is representative of 2 independent experiments.
Slot Blot Apparatus, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
Bio-Rad minitrans blot
Figure 6. (A) Representative Western blot shows vascular endothelial growth factor receptor 2 (VEGFR2) and activated Smad protein expression and (B) proportion of VEGFR2 protein levels in vehicle-treated and 5 ng/mL transforming growth factor beta (TGF-b)-treated bovine aortic endothelial cells (BAECs) cultured in atmospheric (21%) O2 and <0.1% O2 (anoxia) over 6 and 24 hours. Analysis of variance, P ¼ .0015; n ¼ 4; *P < .05. (C) VEGFR2 and Smad protein levels in BAECs treated for 24 hours with 0 to 50 ng/mL bone morphogenetic protein 9 <t>(BMP9)</t> are shown. (D) VEGFR2 and Smad protein levels in BAECs treated for 24 hours with combinations of 10 ng/mL BMP9, 5 ng/mL TGF-b1, and 5 lM SB-431542 (SB) are shown. Western blot is representative of 2 independent experiments.
Minitrans Blot, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Bio-Rad trans-blot cell
Figure 6. (A) Representative Western blot shows vascular endothelial growth factor receptor 2 (VEGFR2) and activated Smad protein expression and (B) proportion of VEGFR2 protein levels in vehicle-treated and 5 ng/mL transforming growth factor beta (TGF-b)-treated bovine aortic endothelial cells (BAECs) cultured in atmospheric (21%) O2 and <0.1% O2 (anoxia) over 6 and 24 hours. Analysis of variance, P ¼ .0015; n ¼ 4; *P < .05. (C) VEGFR2 and Smad protein levels in BAECs treated for 24 hours with 0 to 50 ng/mL bone morphogenetic protein 9 <t>(BMP9)</t> are shown. (D) VEGFR2 and Smad protein levels in BAECs treated for 24 hours with combinations of 10 ng/mL BMP9, 5 ng/mL TGF-b1, and 5 lM SB-431542 (SB) are shown. Western blot is representative of 2 independent experiments.
Trans Blot Cell, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96
Cell Signaling Technology Inc anti total p44 42 mapk
Figure 6. (A) Representative Western blot shows vascular endothelial growth factor receptor 2 (VEGFR2) and activated Smad protein expression and (B) proportion of VEGFR2 protein levels in vehicle-treated and 5 ng/mL transforming growth factor beta (TGF-b)-treated bovine aortic endothelial cells (BAECs) cultured in atmospheric (21%) O2 and <0.1% O2 (anoxia) over 6 and 24 hours. Analysis of variance, P ¼ .0015; n ¼ 4; *P < .05. (C) VEGFR2 and Smad protein levels in BAECs treated for 24 hours with 0 to 50 ng/mL bone morphogenetic protein 9 <t>(BMP9)</t> are shown. (D) VEGFR2 and Smad protein levels in BAECs treated for 24 hours with combinations of 10 ng/mL BMP9, 5 ng/mL TGF-b1, and 5 lM SB-431542 (SB) are shown. Western blot is representative of 2 independent experiments.
Anti Total P44 42 Mapk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Bio-Rad miniprotean tetra system
Figure 6. (A) Representative Western blot shows vascular endothelial growth factor receptor 2 (VEGFR2) and activated Smad protein expression and (B) proportion of VEGFR2 protein levels in vehicle-treated and 5 ng/mL transforming growth factor beta (TGF-b)-treated bovine aortic endothelial cells (BAECs) cultured in atmospheric (21%) O2 and <0.1% O2 (anoxia) over 6 and 24 hours. Analysis of variance, P ¼ .0015; n ¼ 4; *P < .05. (C) VEGFR2 and Smad protein levels in BAECs treated for 24 hours with 0 to 50 ng/mL bone morphogenetic protein 9 <t>(BMP9)</t> are shown. (D) VEGFR2 and Smad protein levels in BAECs treated for 24 hours with combinations of 10 ng/mL BMP9, 5 ng/mL TGF-b1, and 5 lM SB-431542 (SB) are shown. Western blot is representative of 2 independent experiments.
Miniprotean Tetra System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Bio-Rad polyvinylidene diflouride (pvdf) membrane
Figure 6. (A) Representative Western blot shows vascular endothelial growth factor receptor 2 (VEGFR2) and activated Smad protein expression and (B) proportion of VEGFR2 protein levels in vehicle-treated and 5 ng/mL transforming growth factor beta (TGF-b)-treated bovine aortic endothelial cells (BAECs) cultured in atmospheric (21%) O2 and <0.1% O2 (anoxia) over 6 and 24 hours. Analysis of variance, P ¼ .0015; n ¼ 4; *P < .05. (C) VEGFR2 and Smad protein levels in BAECs treated for 24 hours with 0 to 50 ng/mL bone morphogenetic protein 9 <t>(BMP9)</t> are shown. (D) VEGFR2 and Smad protein levels in BAECs treated for 24 hours with combinations of 10 ng/mL BMP9, 5 ng/mL TGF-b1, and 5 lM SB-431542 (SB) are shown. Western blot is representative of 2 independent experiments.
Polyvinylidene Diflouride (Pvdf) Membrane, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96
Bio-Rad mini protean tgx precast gels
Figure 6. (A) Representative Western blot shows vascular endothelial growth factor receptor 2 (VEGFR2) and activated Smad protein expression and (B) proportion of VEGFR2 protein levels in vehicle-treated and 5 ng/mL transforming growth factor beta (TGF-b)-treated bovine aortic endothelial cells (BAECs) cultured in atmospheric (21%) O2 and <0.1% O2 (anoxia) over 6 and 24 hours. Analysis of variance, P ¼ .0015; n ¼ 4; *P < .05. (C) VEGFR2 and Smad protein levels in BAECs treated for 24 hours with 0 to 50 ng/mL bone morphogenetic protein 9 <t>(BMP9)</t> are shown. (D) VEGFR2 and Smad protein levels in BAECs treated for 24 hours with combinations of 10 ng/mL BMP9, 5 ng/mL TGF-b1, and 5 lM SB-431542 (SB) are shown. Western blot is representative of 2 independent experiments.
Mini Protean Tgx Precast Gels, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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99
Bio-Rad mini trans blot apparatus
Figure 6. (A) Representative Western blot shows vascular endothelial growth factor receptor 2 (VEGFR2) and activated Smad protein expression and (B) proportion of VEGFR2 protein levels in vehicle-treated and 5 ng/mL transforming growth factor beta (TGF-b)-treated bovine aortic endothelial cells (BAECs) cultured in atmospheric (21%) O2 and <0.1% O2 (anoxia) over 6 and 24 hours. Analysis of variance, P ¼ .0015; n ¼ 4; *P < .05. (C) VEGFR2 and Smad protein levels in BAECs treated for 24 hours with 0 to 50 ng/mL bone morphogenetic protein 9 <t>(BMP9)</t> are shown. (D) VEGFR2 and Smad protein levels in BAECs treated for 24 hours with combinations of 10 ng/mL BMP9, 5 ng/mL TGF-b1, and 5 lM SB-431542 (SB) are shown. Western blot is representative of 2 independent experiments.
Mini Trans Blot Apparatus, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Rad mini protean trans blot turbo transfer system
Figure 6. (A) Representative Western blot shows vascular endothelial growth factor receptor 2 (VEGFR2) and activated Smad protein expression and (B) proportion of VEGFR2 protein levels in vehicle-treated and 5 ng/mL transforming growth factor beta (TGF-b)-treated bovine aortic endothelial cells (BAECs) cultured in atmospheric (21%) O2 and <0.1% O2 (anoxia) over 6 and 24 hours. Analysis of variance, P ¼ .0015; n ¼ 4; *P < .05. (C) VEGFR2 and Smad protein levels in BAECs treated for 24 hours with 0 to 50 ng/mL bone morphogenetic protein 9 <t>(BMP9)</t> are shown. (D) VEGFR2 and Smad protein levels in BAECs treated for 24 hours with combinations of 10 ng/mL BMP9, 5 ng/mL TGF-b1, and 5 lM SB-431542 (SB) are shown. Western blot is representative of 2 independent experiments.
Mini Protean Trans Blot Turbo Transfer System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Rad mini protean tgx precast polyacrylamide gels
Figure 6. (A) Representative Western blot shows vascular endothelial growth factor receptor 2 (VEGFR2) and activated Smad protein expression and (B) proportion of VEGFR2 protein levels in vehicle-treated and 5 ng/mL transforming growth factor beta (TGF-b)-treated bovine aortic endothelial cells (BAECs) cultured in atmospheric (21%) O2 and <0.1% O2 (anoxia) over 6 and 24 hours. Analysis of variance, P ¼ .0015; n ¼ 4; *P < .05. (C) VEGFR2 and Smad protein levels in BAECs treated for 24 hours with 0 to 50 ng/mL bone morphogenetic protein 9 <t>(BMP9)</t> are shown. (D) VEGFR2 and Smad protein levels in BAECs treated for 24 hours with combinations of 10 ng/mL BMP9, 5 ng/mL TGF-b1, and 5 lM SB-431542 (SB) are shown. Western blot is representative of 2 independent experiments.
Mini Protean Tgx Precast Polyacrylamide Gels, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 6. (A) Representative Western blot shows vascular endothelial growth factor receptor 2 (VEGFR2) and activated Smad protein expression and (B) proportion of VEGFR2 protein levels in vehicle-treated and 5 ng/mL transforming growth factor beta (TGF-b)-treated bovine aortic endothelial cells (BAECs) cultured in atmospheric (21%) O2 and <0.1% O2 (anoxia) over 6 and 24 hours. Analysis of variance, P ¼ .0015; n ¼ 4; *P < .05. (C) VEGFR2 and Smad protein levels in BAECs treated for 24 hours with 0 to 50 ng/mL bone morphogenetic protein 9 (BMP9) are shown. (D) VEGFR2 and Smad protein levels in BAECs treated for 24 hours with combinations of 10 ng/mL BMP9, 5 ng/mL TGF-b1, and 5 lM SB-431542 (SB) are shown. Western blot is representative of 2 independent experiments.

Journal: Cancer

Article Title: Colorectal carcinoma cell production of transforming growth factor beta decreases expression of endothelial cell vascular endothelial growth factor receptor 2.

doi: 10.1002/cncr.26247

Figure Lengend Snippet: Figure 6. (A) Representative Western blot shows vascular endothelial growth factor receptor 2 (VEGFR2) and activated Smad protein expression and (B) proportion of VEGFR2 protein levels in vehicle-treated and 5 ng/mL transforming growth factor beta (TGF-b)-treated bovine aortic endothelial cells (BAECs) cultured in atmospheric (21%) O2 and <0.1% O2 (anoxia) over 6 and 24 hours. Analysis of variance, P ¼ .0015; n ¼ 4; *P < .05. (C) VEGFR2 and Smad protein levels in BAECs treated for 24 hours with 0 to 50 ng/mL bone morphogenetic protein 9 (BMP9) are shown. (D) VEGFR2 and Smad protein levels in BAECs treated for 24 hours with combinations of 10 ng/mL BMP9, 5 ng/mL TGF-b1, and 5 lM SB-431542 (SB) are shown. Western blot is representative of 2 independent experiments.

Article Snippet: BAECs were grown until confluent and serum-starved overnight before treatment in serum-free medium with 0.1 to 20 ng/mL of recombinant human TGF-b1; 10 ng/mL recombinant human BMP9 (both R&D Systems, Minneapolis, Minn); 5 lM SB431542, an inhibitor of TGbR-I isoforms ALK5, ALK4, and ALK722; and 2.5 lM SD-208, an inhibitor of ALK5 and ALK4 (both Tocris Bioscience, Ellisville, Mo).23 CRC cells were grown until superconfluent, serumstarved overnight, then incubated in fresh serum-free DMEM for 48 hours.

Techniques: Western Blot, Expressing, Cell Culture